AmpliGene’s Smart POCT system – AmpEZ is a versatile technology that lends itself for broad range of markets from large clinical laboratory settings, to Point-of-Care (POC).

The technology is ideal for DNA/RNA testing in human in vitro diagnostics (IVD), plant, GMO, Veterinary, Aquaculture, poultry or Biodefence. Studies have indicated that AmpEZ® outperforms other isothermal technologies and overcomes shortcomings of PCR.

AmpEZ® technology answers to increasing demands on faster and more cost-effective testing methods.

The novel AmpEZ® technology

  • Loop Mediated Isotehrmal Amplification (AmpEZ®)
  • Isothermal amplification of a nucleic at a constant Temperature.
  • Both DNA and RNA targets can be detected
  • Proven to work with bacteria, viruses and eukaryotes
  • Only minimal sample preparation is required, no need to extract DNA
  • Ability to multiplex the reaction and detect amplicons individually
  • Features to minimize unspecific amplification
  • Continuous reaction: fast and robust
  • Real-time fluorescent detection, no need to open the amplified tubes, eliminating contamination
  • Easy instrumentation: in the field & POC as well as centralized settings
  • Solid and holistic IPR protection.

AmpEZ®- Benefits

Sensitivity Very low formation of artefacts allows for confidence In positive reactions with low target copies or long Run times
Specificity
  • Anneal curve analysis possible
  • Even 1-2 bp resolution
Speed
  • Rapid reaction
  • Hands-on-time short due to low sample prep requirements
Robustness
  • Tolerate high protein and high salt
  • Temperature exact level not critical
  • Lyophilsed Reagents available.
Multiplexability
  • Multiple different probe/detection chemistry variants developed, some proprietary